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Home›All tests›Vitamins

Vitamin D — 25 Hydroxy

Also known as: vitamin D, 25-OH vitamin D, D3

The standard measure of vitamin D status.

SampleBlood
Report24 hours
FastingNot required
CollectionHome or centre

What’s measured 1 parameters

  • 25-Hydroxy Vitamin D

Why this test is done

Deficiency is very common in Indian adults and causes bone pain, muscle weakness and persistent tiredness.

How to prepare

No fasting needed.

Read more about this

Written by our team and reviewed before publishing.

Vitamin D deficiency is unusually common in India — here is how to knowSunlight is abundant and deficiency is still widespread. What the symptoms actually feel like, which test to ask for, and how to read the number you get back.5 min read

How it works

  1. Pick a 60-minute slot that suits you.
  2. A certified phlebotomist arrives, confirms your OTP, and collects into barcoded tubes.
  3. Your sample is processed under NABL-grade protocols and reviewed by our pathologist.
  4. The doctor-signed report reaches your WhatsApp and your account in 24 hours.

Common questions

Is fasting needed for vitamin D?

No. The sample can be given at any time of day.

What level counts as deficient?

Below 20 ng/mL is generally deficient and 20-29 insufficient, though laboratories differ slightly — compare against the range printed on your report.

How soon should I retest after starting supplements?

Usually about three months, which is long enough to show whether the dose worked.

Do I need to fast before Vitamin D — 25 Hydroxy?

No. Vitamin D — 25 Hydroxy does not require fasting, so you can book any slot that suits you.

How soon will I get the report?

Within 24 hours of collection. It arrives on WhatsApp as a doctor-signed PDF, and a copy stays in your account to download whenever you need it.

Can the sample be collected at home?

Yes, anywhere in our South Delhi service area. Home collection is free on orders above ₹500, and the fee below that is shown before you confirm.

What does Vitamin D — 25 Hydroxy cost?

₹1100. That is the full price — there are no separate reporting or handling charges, and you can pay online or on collection.

Costs less as part of a panel

This test is included in these packages — usually cheaper than booking it alone.

Reliable-1.3₹2750Reliable1.3 GURPAL₹3500Reliable-1.4₹3750Reliable-1.5₹5750

Others also book

Serum Calcium₹1301 paramsVitamin B12₹6001 paramsFolic Acid (Folate)₹8001 params
₹1100₹12008% off
Book home collection
  • Free home collection in South Delhi
  • Report in 24 hours, on WhatsApp
  • Reviewed and signed by our pathologist
  • Pay cash or UPI on collection

Important. This page describes what a test measures and costs. It is not medical advice, and a result needs a doctor to interpret it alongside your symptoms and history. If you have severe symptoms — chest pain, breathlessness, heavy bleeding, fainting or a very high fever — do not wait for a test. Seek emergency care or call 112 immediately.